Heterotrophic growth of Galdieria sulphuraria on residues from aquaculture and fish processing industries

Daniel Pleissner; Stephanie Schönfelder; Nicole Händel; Julia Dalichow; Judith Ettinger; Kristine Kvangarsnes; Egidijus Dauksas; Turid Rustad; Janna Cropotova

doi:10.1016/j.biortech.2023.129281

Heterotrophic growth of Galdieria sulphuraria on residues from aquaculture and fish processing industries

Daniel Pleissner
, Stephanie Schönfelder
, Nicole Händel
, Julia Dalichow
, Judith Ettinger
, Kristine Kvangarsnes
, Egidijus Dauksas
, Turid Rustad
, Janna Cropotova^*

^*Corresponding author for this work

Institute of Sustainable Chemistry

Research output: Journal contributions › Journal articles › Research › peer-review

16 Citations (Scopus)

Abstract

The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 % (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 %, v/v) was selected for upscaling and a biomass concentration of 80 g L⁻¹ (growth rate was 0.72 day⁻¹) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.

Original language	English
Article number	129281
Journal	Bioresource Technology
Volume	384
Number of pages	8
ISSN	0960-8524
DOIs	https://doi.org/10.1016/j.biortech.2023.129281
Publication status	Published - 01.09.2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation
SDG 7 Affordable and Clean Energy
SDG 12 Responsible Consumption and Production
SDG 14 Life Below Water

Research areas and keywords

Algal biomass
Bioeconomy
Bioprocess
Fish processing
Protein residues
Biology

ASJC Scopus Subject Areas

Waste Management and Disposal
Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment

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10.1016/j.biortech.2023.129281Licence: CC BY

Cite this

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title = "Heterotrophic growth of Galdieria sulphuraria on residues from aquaculture and fish processing industries",

abstract = "The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 \% (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 \%, v/v) was selected for upscaling and a biomass concentration of 80 g L−1 (growth rate was 0.72 day−1) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.",

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doi = "10.1016/j.biortech.2023.129281",

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AU - Pleissner, Daniel

AU - Schönfelder, Stephanie

AU - Händel, Nicole

AU - Dalichow, Julia

AU - Ettinger, Judith

AU - Kvangarsnes, Kristine

AU - Dauksas, Egidijus

AU - Rustad, Turid

AU - Cropotova, Janna

PY - 2023/9/1

Y1 - 2023/9/1

N2 - The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 % (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 %, v/v) was selected for upscaling and a biomass concentration of 80 g L−1 (growth rate was 0.72 day−1) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.

AB - The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 % (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 %, v/v) was selected for upscaling and a biomass concentration of 80 g L−1 (growth rate was 0.72 day−1) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.

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KW - Bioeconomy

KW - Bioprocess

KW - Fish processing

KW - Protein residues

KW - Biology

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SN - 0960-8524

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JO - Bioresource Technology

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Heterotrophic growth of Galdieria sulphuraria on residues from aquaculture and fish processing industries

Abstract

Bibliographical note

UN SDGs

Research areas and keywords

ASJC Scopus Subject Areas

Access to Document

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Cite this