Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste

Paula Scarabotto Penteado; Maria Carolina B.Di Medeiros Leal; Maria Gabriela Aparecida Carosio; Alef dos Santos; Mateus Lodi Segatto; Daniel Petinatti Pavarini; Danielle Fernandes da Silva; Jéssica Cristina Amaral; Maria Fátima das G.F. da Silva; Vânia G. Zuin Zeidler; Antonio G. Ferreira

doi:10.3390/foods14040642

Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste

Paula Scarabotto Penteado^*
, Maria Carolina B.Di Medeiros Leal^*
, Maria Gabriela Aparecida Carosio
, Alef dos Santos
, Mateus Lodi Segatto
, Daniel Petinatti Pavarini
, Danielle Fernandes da Silva
, Jéssica Cristina Amaral
, Maria Fátima das G.F. da Silva
, Vânia G. Zuin Zeidler
, Antonio G. Ferreira

^*Corresponding author for this work

Research output: Journal contributions › Journal articles › Research › peer-review

2 Citations (Scopus)

Abstract

Brazil is a global leader in the orange industry, producing approximately one-fourth of the world’s oranges and generating over 50% of the associated waste. These by-products are rich in bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach—microwave-assisted extraction—to recover valuable compounds from orange juice production waste. The extracted compounds were analyzed using nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC–MS). Key bioactives, including D-limonene, valencene, hesperidin, and carbohydrates, were successfully identified. NMR effectively traces and semi-quantifies these compounds, while microwave-assisted extraction enables the sustainable recovery of high-purity hesperidin, confirmed by NMR (87.66%) and HPLC (84.30%) analyses.

Original language	English
Article number	642
Journal	Foods
Volume	14
Issue number	4
Number of pages	14
DOIs	https://doi.org/10.3390/foods14040642
Publication status	Published - 14.02.2025

Bibliographical note

Publisher Copyright:
© 2025 by the authors.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 12 Responsible Consumption and Production
SDG 13 Climate Action

Research areas and keywords

circular economy
environmental health
flavonoids
GC–MS
microwave-assisted extraction
Chemistry

ASJC Scopus Subject Areas

Food Science
Plant Science
Health(social science)
Health Professions (miscellaneous)
Microbiology

Access to Document

10.3390/foods14040642Licence: CC BY

Cite this

@article{00a7ad8c34644935a760445e1e618516,

title = "Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste",

abstract = "Brazil is a global leader in the orange industry, producing approximately one-fourth of the world{\textquoteright}s oranges and generating over 50\% of the associated waste. These by-products are rich in bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach—microwave-assisted extraction—to recover valuable compounds from orange juice production waste. The extracted compounds were analyzed using nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC–MS). Key bioactives, including D-limonene, valencene, hesperidin, and carbohydrates, were successfully identified. NMR effectively traces and semi-quantifies these compounds, while microwave-assisted extraction enables the sustainable recovery of high-purity hesperidin, confirmed by NMR (87.66\%) and HPLC (84.30\%) analyses.",

keywords = "circular economy, environmental health, flavonoids, GC–MS, microwave-assisted extraction, Chemistry",

author = "Penteado, \{Paula Scarabotto\} and Leal, \{Maria Carolina B.Di Medeiros\} and Carosio, \{Maria Gabriela Aparecida\} and \{dos Santos\}, Alef and Segatto, \{Mateus Lodi\} and Pavarini, \{Daniel Petinatti\} and \{da Silva\}, \{Danielle Fernandes\} and Amaral, \{J{\'e}ssica Cristina\} and \{da Silva\}, \{Maria F{\'a}tima das G.F.\} and \{Zuin Zeidler\}, \{V{\^a}nia G.\} and Ferreira, \{Antonio G.\}",

note = "Publisher Copyright: {\textcopyright} 2025 by the authors.",

year = "2025",

month = feb,

day = "14",

doi = "10.3390/foods14040642",

language = "English",

volume = "14",

journal = "Foods",

issn = "2304-8158",

publisher = "MDPI AG",

number = "4",

}

TY - JOUR

T1 - Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste

AU - Penteado, Paula Scarabotto

AU - Leal, Maria Carolina B.Di Medeiros

AU - Carosio, Maria Gabriela Aparecida

AU - dos Santos, Alef

AU - Segatto, Mateus Lodi

AU - Pavarini, Daniel Petinatti

AU - da Silva, Danielle Fernandes

AU - Amaral, Jéssica Cristina

AU - da Silva, Maria Fátima das G.F.

AU - Zuin Zeidler, Vânia G.

AU - Ferreira, Antonio G.

PY - 2025/2/14

Y1 - 2025/2/14

N2 - Brazil is a global leader in the orange industry, producing approximately one-fourth of the world’s oranges and generating over 50% of the associated waste. These by-products are rich in bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach—microwave-assisted extraction—to recover valuable compounds from orange juice production waste. The extracted compounds were analyzed using nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC–MS). Key bioactives, including D-limonene, valencene, hesperidin, and carbohydrates, were successfully identified. NMR effectively traces and semi-quantifies these compounds, while microwave-assisted extraction enables the sustainable recovery of high-purity hesperidin, confirmed by NMR (87.66%) and HPLC (84.30%) analyses.

AB - Brazil is a global leader in the orange industry, producing approximately one-fourth of the world’s oranges and generating over 50% of the associated waste. These by-products are rich in bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach—microwave-assisted extraction—to recover valuable compounds from orange juice production waste. The extracted compounds were analyzed using nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC–MS). Key bioactives, including D-limonene, valencene, hesperidin, and carbohydrates, were successfully identified. NMR effectively traces and semi-quantifies these compounds, while microwave-assisted extraction enables the sustainable recovery of high-purity hesperidin, confirmed by NMR (87.66%) and HPLC (84.30%) analyses.

KW - circular economy

KW - environmental health

KW - flavonoids

KW - GC–MS

KW - microwave-assisted extraction

KW - Chemistry

UR - https://www.scopus.com/pages/publications/85218914150

U2 - 10.3390/foods14040642

DO - 10.3390/foods14040642

M3 - Journal articles

C2 - 40002087

AN - SCOPUS:85218914150

SN - 2304-8158

VL - 14

JO - Foods

JF - Foods

IS - 4

M1 - 642

ER -

Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste

Abstract

Bibliographical note

UN SDGs

Research areas and keywords

ASJC Scopus Subject Areas

Access to Document

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